![]() It has been postulated that while the neutralizing antibodies are essential for controlling SARS-CoV-2 infection, an effective clearance of the virus is dependent on T cells 8, 9, 10, 11. Vaccines elicit humoral and cellular immune responses which work together to protect against infection, in this case SARS-CoV-2 infection 6, 7. Those vaccines have shown immunogenicity, efficacy, and effectiveness against SARS-CoV-2 2, 3, 4, 5. To date, 4 different platforms of SARS-CoV-2 vaccine were labelled as Emergency Use Authorization by WHO, specifically, mRNA vaccines, viral-vector vaccines, protein subunit vaccines, and whole-cell inactivated virus vaccines 1. ![]() There was also a very strong correlation between in-house and commercial SARS-CoV-2 sVNT, a finding of particular interest which will inform future research. This study demonstrated a very strong correlation between in-house ELISA and 2 commercial assays. The optimal cut-off value of S-specific antibodies was 1155.9 BAU/mL, the kappa, sensitivity, and specificity being 0.99, 100%, and 99%, respectively. If using 90% inhibition of sVNT as the reference standard, the optimal cut-off value of RBD-specific antibodies was 442.7 BAU/mL, the kappa, sensitivity, and specificity being 0.99, 99%, and 100%, respectively. The Cohen’s kappa between in-house sVNT and the commercial test was 0.90 (95% CI 0.80, 1.00). The correlation between S-specific binding antibodies and in-house ELISA was 0.96 (95% CI 0.96–0.97) and between RBD-specific antibodies and in-house ELISA was 0.96 (95% CI 0.95–0.97). Three hundred and five samples were included in the analysis. ![]() Samples from healthcare workers who received vaccines against SARS-CoV-2 were tested for RBD-specific antibody, S-specific antibody, and in-house ELISA, commercial sVNT, and in-house sVNT, against wild-type SARS-CoV-2. ![]() This study aimed to evaluate the correlation between in-house and commercial binding-specific IgG antibodies and between in-house and commercial SARS-CoV-2 surrogate virus neutralization tests (sVNT). ![]()
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